Six OECD test guidelines (TGs) have recently been finalised and adopted. OECD TG 402 has been adopted to assess acute dermal toxicity by the fixed dose procedure. The remaining five TGs are used to evaluate eye irritation and serious eye damage potential:

  • Several minor updates have been incorporated in the latest versions of the bovine corneal opacity and permeability test (TG 437) and the isolated chicken eye test (TG 438); these test methods are used to identify chemicals inducing serious eye damage as well as those substances not requiring classification for eye irritation.
  • The Fluorescein Leakage (FL) test (TG 460) is a new in vitro method used to identify ocular corrosives and severe irritants requiring classification in Category 1 for eye effects under EU CLP or GHS.
  • The short time exposure in vitro test method (TG 491) is a new cytotoxicity-based assay using rabbit cornea cells. It can be used to identify substances inducing serious eye damage as well as those not requiring any classification as an eye irritant.
  • TG 405 (in vivo eye irritation testing) was updated to reflect that when testing in animals is still necessary, the use of topical anaesthetics and systemic analgesics is required to reduce or avoid animal pain and distress.

Organisation for Economic Cooperation and Development (2016-2017). Guidelines for the testing of chemicals. Accessible via:


The above items were taken from the December 2017 issue of Toxicology and Regulatory News which is sent automatically to members of bibra.

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