The following four protocols for in vitro toxicity testing have been published on EURL ECVAM’s DB-ALM database, indicated by the European Chemicals Agency as a useful source when considering data gaps for REACH, or in a weight of evidence approach:

  • EpiSkin™ skin irritation test
  • GreenScreen HC™ genotoxicity test
  • Human whole blood/(IL)-1β in vitro pyrogenicity test 
  • Human cryopreserved whole blood/(IL)-1β in vitro pyrogenicity test

A further skin irritation test protocol, SKINETHIC™, was apparently due for release in July 2012, but was not published at the time of printing.

European Reference Laboratory for Alternatives to Animal testing. Database Service on Alternative Methods to Animal Experimentation. Protocol numbers 131-135. (select “methods search” option, then “INVITTOX protocols”. Free registration is required to access these documents)
Also see


The above item was taken from the September 2012 issue of Toxicology and Regulatory News which is sent automatically to members of bibra (click here for more details).

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