In February 2015, OECD adopted two novel skin sensitisation TGs. The in chemico direct peptide reactivity assay (DPRA) (TG 442C) measures a test chemical’s potential to bind to proteins, while the in vitro ARE-Nrf2 luciferase test method (TG 442D) assesses the activation of human keratinocytes (found in the epidermis). Covalent binding to proteins and keratinocyte activation are considered to be two key events in the adverse outcome pathway (AOP) for skin sensitisation.
Organisation for Economic Co-operation and Development (2015). Guidelines for the testing of chemicals. Available via http://www.oecd-ilibrary.org/environment/oecd-guidelines-for-the-testing-of-chemicals-section-4-health-effects_20745788
The above items were taken from the March 2015 issue of Toxicology and Regulatory News which is sent automatically to members of bibra (click here)
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